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Practical HPLC Method Development Book

Practical HPLC Method Development
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Practical HPLC Method Development, This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of , Practical HPLC Method Development
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  • Practical HPLC Method Development
  • Written by author Lloyd R. Snyder
  • Published by Wiley, John & Sons, Incorporated, 12/3/2012
  • This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of
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Preface
Glossary of Symbols and Terms
1 Getting Started 1
2 Basics of Separation 21
3 Detection Sensitivity and Selectivity 59
4 Sample Preparation 100
5 The Column 174
6 Non-ionic Samples: Reversed- and Normal-Phase HPLC 233
7 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC 292
8 Gradient Elution 350
9 Systematic Approach to the Reversed-Phase Separation of Regular Samples 402
10 Computer-Assisted Method Development 439
11 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds 479
12 Chiral Separations 537
13 Preparative HPLC Separation 616
14 Quantitation (Including Trace Analysis) 643
15 Completing the Method: Validation and Transfer 685
App. I Plate Number and Resolution 714
App. II Properties of Solvents Used in HPLC 721
App. III Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure 729
App. IV Preparing Buffered Mobile Phases 735
App. V Characterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers 740
App. VI Adjusting Mobile-Phase Water Content for Normal-Phase HPLC 744
Index 747


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Practical HPLC Method Development, This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of , Practical HPLC Method Development

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Practical HPLC Method Development, This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of , Practical HPLC Method Development

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Practical HPLC Method Development, This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of , Practical HPLC Method Development

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