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Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants. DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.
Henderson (U. of Dundee, UK) and his team of researchers provide instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. Topics of the 52 chapters include how to screen for DNA repair mutants in genetically accessible (mostly mammalian) model systems; how cDNAs of human repair genes can be used for phenotypic correction of repair- deficient cells; methodologies for characterizing repair proteins and for assessing levels of DNA damage and repair; methods for inducing double strand breaks in DNA and/or measuring their repair; and methods for studying "physiological" responses to DNA injury. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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