Mass Spectrometry in Biology and Medicine Book

	Organizing Committee/Sponsors
	Preface
	A New Delayed Extraction MALDI-TOF MS-MS for Characterization of Protein Digests	1
	Measurements of Protein Structure and Noncovalent Interactions by Time-of-Flight Mass Spectrometry With Orthogonal Ion Injection	17
	Isotopic Amplification, H/D Exchange, and Other Mass Spectrometric Strategies for Characterization of Biomacromolecular Topology and Binding Sites	31
	Probing the Nature of Amyloidogenic Proteins by Mass Spectrometry	53
	Studying Noncovalent Small Molecule Interactions with Protein and RNA Targets by Mass Spectrometry	73
	Identification of Protein-Protein Interfaces by Amide Proton Exchange Coupled to MALDI-TOF Mass Spectrometry	91
	Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation	111
	Protein Micro-Characterization by Mass Spectrometry: Sample Handling and Data Flow	121
	Towards an Integrated Analytical Technology for the Generation of Multidimensional Protein Expression Maps	143
	Sequencing the Primordial Soup	163
	Coaxial Nanospray Coupled with a Hybrid Quadrupole/Time-of-Flight Tandem Mass Spectrometer for Proteome Studies	179
	Life Without Databases: De Novo Sequencing of Small Gene Products and Complete Characterization of Posttranslational Modifications	199
	Investigation of Intact Subunit Polypeptide Composition of the 20S Proteasome Complex from Rat Liver Using Mass Spectrometry	217
	Deciphering Functionally Important Multiprotein Complexes by Mass Spectrometry	237
	Investigation of Apoptosis-Involved Processes by Mass Spectrometric Identification of the Apoptosis-Associated Proteins in IgM-Induced Burkitt Lymphoma Cells	271
	IR-MALDI - Softer Ionization in MALDI-MS for Studies of Labile Macromolecules	289
	Identification of in-vivo Phosphorylation Sites with Mass Spectrometry	309
	Determination of Enzyme Mechanisms by Stopped-Flow Electrospray Mass Spectrometry	329
	Glycosylation of Proteins - A Major Challenge in Mass Spectrometry and Proteomics	365
	Site-specific Characterization of the N-linked Glycans of Murine PrP[superscript Sc] Using Advanced Methods of Electrospray Mass Spectrometry	383
	Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of N-Linked Carbohydrates and Related Compounds	403
	Electrophoretic and Mass Spectrometric Strategies for the Identification of Lipopolysaccharides and Immunodeterminants in Pathogenic Strains of Haemophilus influenzae; Application to Clinical Isolates	439
	Mycobacterial Lipoglycans: Structure and Roles in Mycobacterial Immunity	463
	The Impact of Drug Metabolism in Contemporary Drug Discovery: New Opportunities and Challenges for Mass Spectrometry	481
	Electrospray Mass Spectrometric Analysis of Lipid Mediators Derived from Arachidonic Containing Membrane Phospholipids	497
	Mass Spectrometry After the Human Genome Project	515
	Problems and Prospects in the Characterization of Posttranscriptional Modifications in Large RNAs	531
	Appendix	553
	Author Index	563
	Subject Index	565