Parkinson's Disease: Methods and Protocols Book

Part I. Genetics

Point Mutations in the _-Synuclein Gene

Abbas Parsian and Joel S. Perlmutter

Autosomal Recessive Juvenile Parkinsonism (AR-JP): Genetic Diagnosis

Hiroto Matsumine, Nobutaka Hattori, and Yoshikuni Mizuno

Part II. Molecular Pathogenetic Studies

Parkinson's Disease, Dementia with Lewy Bodies, and Multiple System Atrophy as _-Synucleinopathies

Michel Goedert, Ross Jakes, R. Anthony Crowther, and Maria Grazia Spillantini

_-Synuclein/Amyloid Interactions

Poul Henning Jensen

Functional Defect Conferred by the Parkinson's Disease-Causing _-Synuclein (Ala30Pro) Mutation

Poul Henning Jensen

Molecular Biology of Dopamine-Induced Apoptosis: Possible Implications for Parkinson's Disease

Ilan Ziv, Anat Shirvan, Daniel Offen, Ari Barzilai, and Eldad Melamed

Animal Models of Induced Apoptotic Death in the Substantia Nigra

Tinmarla F. Oo and Robert E. Burke

Apoptotic Morphology in Phenotypically Defined Dopaminergic Neurons of the Substantia Nigra

Tinmarla F. Oo and Robert E. Burke

The Role of Nitric Oxide in Parkinson's Disease

Serge Przedborski and Ted M. Dawson

Oxidative Stress Indices in Parkinson's Disease: Biochemical Determination

Moussa B. H. Youdim, Noam Drigues, and Silvia Mandel

Part III. Molecular Aspects of Basal Ganglia Function

Quantification of Tyrosine Hydroxylase mRNA

Hiroshi Ichinose, Tamae Ohye, Takahiro Suzuki, Hidehito Inagaki, and Toshiharu Nagatsu

Immunochemical Analysis of Dopamine Transporters in Parkinson's Disease

Gary W. Miller and Allan I. Levey

Dopamine Transporter and Vesicular Monoamine Transporter Knockout Mice: Implications for Parkinson's Disease

Gary W. Miller, Yan-Min Wang, Raul R. Gainetdinov, and Marc G. Caron

Transcription Mechanisms for Dopamine Receptor Genes

Sang-Hyeon Lee and M. Maral Mouradian

Detection of DNA/Protein Interactions in Dopamine Receptor Genes

Sang-Hyeon Lee and M. Maral Mouradian

In Situ Hybridization on Brain Tissue

Claas-Hinrich Lammers, Yoshinobu Hara, and M. Maral Mouradian

Regulation of Striatal N-Methyl-d-Aspartate Receptor (NMDAR) Function by Phosphorylation of Its Subunits in Parkinsonian Rats

Justin D. Oh

Part IV. Molecular Therapies

Chronic Intracerebral Delivery of Trophic Factors via a Programmable Pump as a Treatment for Parkinsonism

Richard Grondin, Zhiming Zhang, Dennis D. Elsberry, Greg A. Gerhardt, and Don M. Gash

Grafting Genetically Engineered Cells into the Striatum of NonHuman Primates

Krys S. Bankiewicz, Phillip Pivirotto, Rosario Sanchez-Pernaute, and Eugene O. Major

Encapsulated Cell Implants as a Novel Treatment for Parkinson's Disease

Jack L. Tseng and Patrick Aebischer

Neural Stem Cell Technology as a Novel Treatment for Parkinson's Disease

Richard J. E. Armstrong, Anne E. Rosser, Stephen B. Dunnett, and Roger A. Barker