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Preface | v | |
Contributors | xi | |
1 | Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity | 1 |
2 | Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue | 21 |
3 | Confocal Microscopy of Adipocytes | 53 |
4 | Cellularity Measurements | 65 |
5 | Subcellular Fractionation of Adipocytes and 3T3-L1 Cells | 77 |
6 | Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies | 83 |
7 | Glycogen Synthase Activity in Adipose Tissue: Methods for Freeze-Clamping and Assay | 89 |
8 | Assays of Lipolytic Enzymes | 97 |
9 | Assays of Lipogenic Enzymes | 121 |
10 | Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements | 129 |
11 | Transfection of Adipocytes and Preparation of Nuclear Extracts | 141 |
12 | Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes | 147 |
13 | Assays of Glucose Entry, Glucose Transporter Amount, and Translocation | 157 |
14 | Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes | 167 |
15 | Measurements of Glucose Conversion to its Metabolites | 181 |
16 | Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes | 193 |
17 | Culture of Adipose Tissue and Isolated Adipocytes | 197 |
18 | Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines | 213 |
19 | Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue | 225 |
20 | Cultures of Human Adipose Precursor Cells | 239 |
21 | Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes | 249 |
22 | Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences | 269 |
23 | Measurement of Adipose Tissue Blood Flow | 281 |
24 | Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue | 295 |
25 | Measurements of White Adipose Tissue Metabolism by the Microdialysis Technique | 305 |
26 | Immunoassay Measurements of Leptin | 323 |
Index | 331 |
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Add Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols to the inventory that you are selling on WonderClubX
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Add Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols to your collection on WonderClub |