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Adipose Tissue Protocols Book

Adipose Tissue Protocols
Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols has a rating of 3 stars
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Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols
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  • Adipose Tissue Protocols
  • Written by author Gerard Ailhaud
  • Published by Springer-Verlag New York, LLC, March 2001
  • Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a
  • Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods c
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Authors

Prefacev
Contributorsxi
1Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity1
2Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue21
3Confocal Microscopy of Adipocytes53
4Cellularity Measurements65
5Subcellular Fractionation of Adipocytes and 3T3-L1 Cells77
6Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies83
7Glycogen Synthase Activity in Adipose Tissue: Methods for Freeze-Clamping and Assay89
8Assays of Lipolytic Enzymes97
9Assays of Lipogenic Enzymes121
10Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements129
11Transfection of Adipocytes and Preparation of Nuclear Extracts141
12Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes147
13Assays of Glucose Entry, Glucose Transporter Amount, and Translocation157
14Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes167
15Measurements of Glucose Conversion to its Metabolites181
16Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes193
17Culture of Adipose Tissue and Isolated Adipocytes197
18Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines213
19Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue225
20Cultures of Human Adipose Precursor Cells239
21Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes249
22Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences269
23Measurement of Adipose Tissue Blood Flow281
24Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue295
25Measurements of White Adipose Tissue Metabolism by the Microdialysis Technique305
26Immunoassay Measurements of Leptin323
Index331


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Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols

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Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols

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Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a , Adipose Tissue Protocols

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