Herpes Simplex Virus Protocols, Vol. 10 Book

	Preface
	Contributors
1	HSV Growth, Preparation, and Assay	1
2	HSV Entry and Spread	9
3	Preparation of HSV-DNA and Production of Infectious Virus	19
4	HSV Mutagenesis	27
5	Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using in Vivo Reconstitution of Virus from Cloned Subgenomic Regions	43
6	Construction and Use of Cell Lines Expressing HSV Genes	89
7	Analysis of HSV Polypeptides	97
8	Protein Purification	121
9	Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells	131
10	Crystallization of Macromolecules for Three-Dimensional Structure Determination	157
11	Direct Immunogold Labeling of Herpesvirus Suspensions	173
12	Expression of HSV Proteins in Bacteria	185
13	In Vitro Systems to Analyze HSV Transcript Processing	193
14	Analysis of HSV-1 Transcripts by RNA-PCR	205
15	Transient Assays for HSV Origin and Replication Protein Function	215
16	HSV Amplicons in Gene Therapy	227
17	Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions	237
18	HSV-Cellular Protein Interactions	257
19	Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse	273
20	Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit	291
21	HSV Latency In Vitro: In Situ Hybridization Methods	317
22	Assessing Cell-Mediated Immune Responses to HSV in Murine Systems	327
23	Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection	345
24	Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction	355
25	HSV Vectors for Gene Therapy	369
26	Investigation of the Anti-HSV Activity of Candidate Antiviral Agents	387
	Index	407