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Preface vii
An Important Message About Safety ix
Basic Laboratory Techniques and Skills 1
Culture Transfer Techniques 5
Aseptic Transfer Techniques 5
Pure Culture Techniques 11
Streak Plate Technique 11
Pour Plate Technique 14
Microscopy and Bacterial Staining Techniques 21
The Compound Microscope 23
Parts of the Microscope and Their Use 23
Observations of Prepared Slides 25
Preparation of a Bacterial Smear and the Simple Stain Technique 35
Preparation of a Bacterial Smear 35
The Simple Stain Technique 38
The Negative Stain Technique 43
The Gram Stain Technique 49
Bacterial Structures 55
Spore Stain Technique 55
Capsule Stain Technique 57
Bacterial Motility 59
Viruses and Eukaryotic Microorganisms 65
Viruses 67
Constructing a Virus 67
The Effect of Bacteriophages on Bacteria 68
Plaque Formation 70
Identification of Bacteriophages from Sewage 72
Estimating the Number of Bacteriophages 72
Fungi, Protozoa, and Multicellular Parasites 79
Fungi (Molds and Yeasts) 79
Protozoa and Multicellular Parasites 84
The Spread and Control of Microorganisms 91
A Simulated Epidemic 93
Microbial Transmission via Fomites 93
A Microbial Hunt 95
Transmission of Microorganisms through Toilet Paper 97
The Effect of Physical Agents on Bacteria 101
Heat Tolerance 101
Ultraviolet Light 103
Drying 105
Other Physical Agents 106
The Effect of Chemical Agents on Bacteria 111
Antiseptics and Disinfectants 111
Metals 113
Other Chemical Substances 114
Effect of Lysozyme on Bacteria 115
Disinfection of Drinking Water 116
Evaluation of Disinfectants and Antiseptics 125
Effectiveness of Disinfectants on Inanimate Objects 125
Effectiveness of Disinfectants and Hand Washing on the Skin Surface 126
Mouthwashes and Oral Bacteria 127
The Phenol Coefficient 128
The Effect of Antibiotics on Bacteria 135
Microbial Growth: Analysis of a Bacterial Growth Curve 145
Bacterial Growth Dynamics 146
Plotting a Growth Curve and Determining the Generation Time 147
Medical Microbiology 151
The Genus Mycobacterium 153
Colony and Cellular Morphology 153
Acid-Fast Stain Technique 154
Cold Acid-Fast Stain Technique 156
The Genus Streptococcus 159
Streptococci from the Upper Respiratory Tract 159
Streptococci from the Oral Cavity 161
The Genus Neisseria 167
The Genus Staphylococci 171
Isolation of Staphylococcus 171
Differentiation between Staphylococci Species 173
The Enteric Bacteria 179
Isolation of Enteric Bacteria 179
Differentiation of Enteric Bacteria on TSI Agar 180
The IMViC Series 182
Rapid Identification of Enteric Bacteria 183
The Genus Bacillus 191
The Genus Clostridium 195
The Genus Lactobacillus 201
Isolation of Lactobacilli 201
Caries Susceptibility Test 202
Identification of a Bacterial Unknown 207
Bacterial Structural Characteristics 213
Bacterial Culture Characteristics 217
Biochemical Characteristics of Bacteria 223
Carbohydrate Fermentation 223
Starch Digestion 224
Catalase Production 226
DNA Digestion 227
Hydrogen Sulfide Production 228
IMViC Series 229
Urea Digestion 230
Lipid Digestion 230
Casein Digestion 231
Bacterial Genetics and Serology 239
Bacterial Genetics: Mutations and the Ames Test 241
Gradient Plate Technique 242
Mutagenic Effect of Ultraviolet Light 243
DNA Repair of UV Light Damage 245
The Ames Test 246
Bacterial Conjugation 253
Bacterial Transformation 259
Serology: Agglutination Techniques and Blood Cell Identification 267
Slide Agglutination 267
Tube Agglutination 268
Hemagglutination: Determination of Blood Type 270
Blood Smear 272
Public Health and Environmental Microbiology 277
Microbiology of Foods 279
Food Preservation with Salt and Garlic 279
Standard Plate Count of Food Products 280
Fermentation of Sauerkraut 283
Fermentation of Wine and Beer 284
Microbiology of Milk and Dairy Products 293
Standard Plate Count of Milk 293
Coliform Plate Count of Milk 295
Methylene Blue Reduction Test 296
Preparation of Cheese 297
Preparation of Yogurt 298
Natural Bacterial Content of Milk 299
Microbiology of Water 307
Presumptive Test by the MPN Method 307
Confirmed Test 308
Completed Test 310
Membrane Filter Technique 311
Preparation of a Biofilm 312
Microbiology of Soil 319
Isolation of Rhizobium from Legume Roots 319
Ammonification by Soil Microorganisms 320
Isolation of Streptomyces from Soil 321
Antibiotic Production by Streptomyces 321
Plate Count of Soil Bacteria 323
Microbial Ecology in the Soil-The Winogradsky Column 323
Recycling of Carbon in Food 325
Recycling of Carbon in Cloth 327
Recycling of Carbon in Leaves 328
Preparation of Stains and Diagnostic Reagents 337
Using Enterotube II 339
Index 343
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Add Alcamo's Laboratory Fundamentals of Microbiology - 9th Edition, This popular lab manual offers thirty-four multi-part lab exercises designed to provide students with basic training in the handling of microorganisms, while exploring microbial properties and uses. This lab manual can also be used independently of the ma, Alcamo's Laboratory Fundamentals of Microbiology - 9th Edition to the inventory that you are selling on WonderClubX
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Add Alcamo's Laboratory Fundamentals of Microbiology - 9th Edition, This popular lab manual offers thirty-four multi-part lab exercises designed to provide students with basic training in the handling of microorganisms, while exploring microbial properties and uses. This lab manual can also be used independently of the ma, Alcamo's Laboratory Fundamentals of Microbiology - 9th Edition to your collection on WonderClub |