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Foreword | ||
Preface | ||
1 | Introduction | 1 |
1.1 | Development of Capillary Electrophoresis | 1 |
1.2 | Capillary Electrophoresis System | 9 |
1.3 | Comparison of Capillary Electrophoresis to Other Separation Techniques | 14 |
1.4 | Summary of the Principle Features of Capillary Electrophoresis | 17 |
2 | Principles of Separation | 19 |
2.1 | Electroosmotic Flow | 19 |
2.2 | Electrophoretic Mobility | 39 |
2.3 | Effects of Electrophoretic Parameters on Separation Parameters | 44 |
3 | Modes of Capillary Electrophoresis | 53 |
3.2 | Capillary Zone Electrophoresis | 54 |
3.3 | Micellar Electrokinetic Capillary Chromatography (MEKC) | 55 |
3.4 | Capillary Gel Electrophoresis (CGE) | 74 |
3.5 | Capillary Isoelectric Focusing (CIEF) | 80 |
3.6 | Capillary Isotachophoresis (CITP) | 84 |
3.7 | Summary of Modes of Capillary Electrophoresis | 90 |
4 | Instrumental Considerations | 94 |
4.1 | Capillary Electrophoresis System | 94 |
4.2 | Sample Injection | 95 |
4.3 | Detection | 113 |
4.4 | High-Voltage Power Supply | 141 |
4.5 | Capillary Temperature Control | 142 |
4.6 | Source and Destination Vials and Buffer Replenishment | 146 |
4.7 | Fraction Collection | 148 |
4.8 | Capillaries | 152 |
4.9 | Automatic Sampling and Instrument Control | 154 |
5 | Developing a Method | 159 |
5.2 | Selection of the Mode of Capillary Electrophoresis | 164 |
5.3 | Capillary | 169 |
5.4 | Run Buffer Selection | 175 |
5.5 | Voltage | 183 |
5.6 | Sample Pretreatment | 183 |
5.7 | Injection Mode and Amount | 184 |
5.8 | Capillary Temperature | 186 |
5.9 | Detector Selection | 186 |
5.10 | Buffer Replenishment | 188 |
5.11 | Data Reporting | 189 |
6 | Qualitative and Quantitative Analysis | 193 |
6.2 | Qualitative Analysis | 194 |
6.3 | Quantitative Analysis | 200 |
7 | Applications | 211 |
7.2 | Inorganic Anions | 211 |
7.3 | Inorganic Cations | 219 |
7.4 | Chiral Separations | 219 |
7.5 | Proteins | 222 |
7.6 | Nucleic Acids, Oligonucleotides | 227 |
7.7 | Sampling from Single Cells | 228 |
7.8 | Determination of Diffusion Coefficients | 228 |
Index | 237 |
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Add Capillary Electrophoresis, The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is, Capillary Electrophoresis to the inventory that you are selling on WonderClubX
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Add Capillary Electrophoresis, The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is, Capillary Electrophoresis to your collection on WonderClub |