Biomarkers In Marine Organisms Book

	Preface
	Contents
	List of Participants of European Projects
	Abbreviations
Ch. 1	Induction of Molluscan Cytochrome P450 Monooxygenase System as a Biomarker of Organic Pollution in Environmental Monitoring	1
Ch. 2	Sensitivity and Specificity of Metallothionein as a Biomarker for Aquatic Environment Biomonitoring	29
Ch. 3	Genotoxicity Biomarkers in Aquatic Organisms as Indictors of Carcinogenic Marine Pollutants	45
Ch. 4	DNA Adduct Detection in Mussels Exposed to Bulky Aromatic Compounds in Laboratory and Field Conditions	65
Ch. 5	Developmental, Cytogenetic and Biochemical Effects of Spiked or Environmentally Polluted Sediments in Sea Urchin Bioassays	85
Ch. 6	Comparative Study of Sediment and Mussel Aromatic Compound Content in European Coastal Environments. Relationship with Specific Biomarkers	131
Ch. 7	Monitoring of Biological Effects of Pollutants: Field Application	179
Ch. 8	Biochemical Markers in Mussel, Mytilus sp., and Pollution Monitoring in European Coasts: Data Analysis	215
Ch. 9	Investigation of Genotoxicity and Immunotoxicity for Monitoring Marine Pollution in the Baltic Sea and Mediterranean Sea	237
Ch. 10	Biochemical Tools for the Assessment of Pesticide Exposure in a Deltaic Environment: The Use of Cholinesterases and Carboxylesterases	259
Ch. 11	Environmental Monitoring in the North Sea by Combining Biomarker Studies in the Sea Star Asterias Rubens with Sediment Quality Assessment Based on Sea Urchin Bioassays	279
Ch. 12	Cholinesterase Activity as a Bioindicator for Monitoring Marine Pollution in the Baltic Sea and the Mediterranean Sea	331
Ch. 13	Evaluation of Various Biomarkers in the Wild Fish Serranus Cabrilla Collected in the NW Mediterranean Sea	343
Ch. 14	Microbiological Indicators for Monitoring Marine Pollution in the Baltic Sea and the Mediterranean Sea	357
Ch. 15	Isolation of Cytochrome P450 CDNAS (CYP1A1 and CYP4T2) from the Sea Bass (Dicentrarchus Labrax): Tools for Biomonitoring Sea Pollution	367
Ch. 16	Inhibitory Effects of Heavy Metals on CYP1A1 Induction in Black Seabream (Spondyliosoma Cantharus) Hepatocyte Cultures	393
Ch. 17	Biochemical Responses of Crabs (Carcinus spp) to Polycyclic Aromatic Hydrocarbons (PAHs) as the Basis for New Biomarker Assays	409
Ch. 18	Cloning of Metallothionein cDNAS in Carcinus Maenas	431
Ch. 19	Development of Cytochrome P450 Biomarkers from Posidinia Oceanica	439
	Technical Annexes	447
	Measurement of Total Cytochrome P450 Content in Digestive Gland Microsomes of Mussel (Mytilus sp.)	449
	Measurement of Cytochrome P450 Immunopositive Proteins in Digestive Gland Microsomes of Mussel (Mytilus sp.) by Western Analysis	451
	Metallothionein Assay	453
	Evaluation of Lysosomal Membrane Stability	455
	Alkaline Filter Elution Method	461
	Mussel Micronucleus Test	467
	Detection of Bulky Aromatic DNA Adducts by [superscript 32]P-postlabelling	471
	Embryotoxicity on Sea Urchins	475
	Sediment Spiking Technique	477
	Measurement of Oxidative Activity: Luminol-dependent Chemiluminescence and 8-Oxodesoxyguanine	478
	Chemical Analyses of PAH in Sediment and Mussels	481
	Procedures for Cholinesterase Determination in Fish and Mussel	487
	Microplate Method for Measurement of EthoxyResorufin-O-Deethylase (EROD) in Fish	491
	Benzo(a)pyrene Hydroxylase Activity Measurement in Microsomes from Mytilus sp	495
	Determination of Cholinesterase Activity in Mussel Gills	499
	Chemical Analysis: PAHs, PCBs and Pesticides	501
	Biochemical Markers Acetylcholinesterase Activity and Cytochrome P450	503
	Measurement of DNA-Strandbreaks in Tissue of the Sea Star Asterias Rubens	505
	Measurement of Sediment-Toxicity Using a Fertilization and Embryonic Development Assay with the Sea Urchins (Psammechinus Miliaris, Paracentrotus Lividus)	509
	Determination of EROD, GST and AchE Activities in Painted Comber (Serranus Cabrilla)	513
	Miniaturized DNA Unwinding Assay Employing Hydroxyapatite "Batch" Elution	517
	Molecular Cloning of CYP Genes in the Sea Bass (Dicentrarchus Labrax): Experimental Procedures	519
	Fish Hepatocyte Cultures	527
	Determination of Cytochromes P450 Expression and Induction in Black Seabream (Spondyliosama cantharus): Enzyme Activities, Northern and Esters Blots	529
	Biochemical Responses of Crabs (Carcinus spp) to Polycyclic Aromatic Hydrocarbons (PAHs) as the Basis for New Biomarkers Assays	533
	Cloning of Metallothionein cDNAs in Carcinus Maenas	537
	Development of Biomarkers from Seagrass Posidonia Oceanica	539
	List of Contributors	543
	Index of Keywords	549