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Enzyme Functionality: Design, Engineering And Screening Book

Enzyme Functionality: Design, Engineering And Screening
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Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening
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  • Enzyme Functionality: Design, Engineering And Screening
  • Written by author Edited by Allan Svendsen
  • Published by Taylor & Francis, Inc., 10/28/2003
  • Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu
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Authors

Preface
Contributors
1 Concepts for Protein Engineering 1
2 Sequence Families and Modular Organization of Carbohydrate-Active Enzymes 15
3 Analyzing Three-Dimensional Structures of Variant Enzymes 35
4 Quantitative Modeling of Lipase Enantioselectivity 59
5 Rational Redesign of Haloalkane Dehalogenases Guided by Comparative Binding Energy Analysis 79
6 Computer Simulations: A Tool for Investigating the Function of Complex Biological Macromolecules 97
7 Calculations of Ionization Equilibria in Proteins 149
8 Modeling and Optimization of Directed Evolution Protocols 185
9 Rational Redesign of Enzymes 213
10 Details in the Reaction Mechanism of Chitinases
11 Kinetic Evolution to the Catalytic Core of the Bacterial Phosphotriesterase 247
12 Protein Engineering of PQQ Glucose Dehydrogenase 261
13 The Proline Rule: A Concept for Engineering Protein Stability 293
14 Homing Endonucleases: Tools and Targets for Protein Engineering 325
15 Evolutionary Methods for Protein Engineering 353
16 Directed Evolution by Random Mutagenesis: A Critical Evaluation 375
17 Enzyme Engineering by Phage Display 391
18 In Vivo Gene Shuffling in Yeast: A Fast and Easy Method for Directed Evolution of Enzymes 413
19 Effective DNA Shuffling Methods for Enzyme Evolution 425
20 Exploring the Functional Space of Combinatorial Mutant Libraries for the Directed Evolution of Novel Enzyme Activities 443
21 Modifying the Character of an Enzyme by Producing Chimeric Enzymes: Chimeric [beta]-glucosidases as an Illustration 461
22 Assay Systems for Screening or Selection of Biocatalysts 475
23 Screening of Enzyme Variants for Thermostability 491
24 Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution 507
25 Screen Automation and Robotics 525
26 Screening for Enantioselective Enzymes 559
27 Enzyme Engineering by Microbial Cell Surface Display 599
28 Overexpression and Secretion of Biocatalysts in Pseudomonas 617
29 Analysis of Catalytic and Structural Stability of Native and Covalently Modified Enzymes 633
Index 661


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Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening

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Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening

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Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening

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