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Preface | ||
Contributors | ||
1 | Concepts for Protein Engineering | 1 |
2 | Sequence Families and Modular Organization of Carbohydrate-Active Enzymes | 15 |
3 | Analyzing Three-Dimensional Structures of Variant Enzymes | 35 |
4 | Quantitative Modeling of Lipase Enantioselectivity | 59 |
5 | Rational Redesign of Haloalkane Dehalogenases Guided by Comparative Binding Energy Analysis | 79 |
6 | Computer Simulations: A Tool for Investigating the Function of Complex Biological Macromolecules | 97 |
7 | Calculations of Ionization Equilibria in Proteins | 149 |
8 | Modeling and Optimization of Directed Evolution Protocols | 185 |
9 | Rational Redesign of Enzymes | 213 |
10 | Details in the Reaction Mechanism of Chitinases | |
11 | Kinetic Evolution to the Catalytic Core of the Bacterial Phosphotriesterase | 247 |
12 | Protein Engineering of PQQ Glucose Dehydrogenase | 261 |
13 | The Proline Rule: A Concept for Engineering Protein Stability | 293 |
14 | Homing Endonucleases: Tools and Targets for Protein Engineering | 325 |
15 | Evolutionary Methods for Protein Engineering | 353 |
16 | Directed Evolution by Random Mutagenesis: A Critical Evaluation | 375 |
17 | Enzyme Engineering by Phage Display | 391 |
18 | In Vivo Gene Shuffling in Yeast: A Fast and Easy Method for Directed Evolution of Enzymes | 413 |
19 | Effective DNA Shuffling Methods for Enzyme Evolution | 425 |
20 | Exploring the Functional Space of Combinatorial Mutant Libraries for the Directed Evolution of Novel Enzyme Activities | 443 |
21 | Modifying the Character of an Enzyme by Producing Chimeric Enzymes: Chimeric [beta]-glucosidases as an Illustration | 461 |
22 | Assay Systems for Screening or Selection of Biocatalysts | 475 |
23 | Screening of Enzyme Variants for Thermostability | 491 |
24 | Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution | 507 |
25 | Screen Automation and Robotics | 525 |
26 | Screening for Enantioselective Enzymes | 559 |
27 | Enzyme Engineering by Microbial Cell Surface Display | 599 |
28 | Overexpression and Secretion of Biocatalysts in Pseudomonas | 617 |
29 | Analysis of Catalytic and Structural Stability of Native and Covalently Modified Enzymes | 633 |
Index | 661 |
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Add Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening to the inventory that you are selling on WonderClubX
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Add Enzyme Functionality: Design, Engineering And Screening, Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolu, Enzyme Functionality: Design, Engineering And Screening to your collection on WonderClub |