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Book Categories |
List of Contributors | ||
Preface | ||
Contents of Methods in Enzymology, Volumes 63, 64, 87, and 249 | ||
1 | Transient Kinetic Approaches to Enzyme Mechanisms | 1 |
2 | Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays | 37 |
3 | Derivation of Initial Velocity and Isotope Exchange Rate Equations | 57 |
4 | Plotting Methods for Analyzing Enzyme Rate Data | 89 |
5 | Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models | 111 |
6 | Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects | 133 |
7 | Product Inhibition Applications | 183 |
8 | Reversible Enzyme Inhibitors as Mechanistic Probes | 207 |
9 | Transition State and Multisubstrate Analog Inhibitors | 229 |
10 | Application of Affinity Labeling for Studying Structure and Function of Enzymes | 259 |
11 | Mechanism Based Enzyme Inactivators | 291 |
12 | Isotope Exchange Methods for Elucidating Enzymic Catalysis | 335 |
13 | Positional Isotope Exchange as Probe of Enzyme Action | 379 |
14 | Isotope Effects: Determination of Enzyme Transition State Structure | 407 |
15 | Hydrogen Tunneling in Enzyme Catalysis | 441 |
16 | Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis | 467 |
17 | Selected Exercises and Problems | 497 |
Index | 531 |
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