Lipids Book

Membrane Dynamics and Reconstitution Assays:

1. Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding
2. Studying lipids involved in the endosomal pathway
3. Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking
4. Reconstituting Multivesicular Body Biogenesis with purified components
5. Approaches to the study of Atg8-mediated membrane dynamics in vitro
6. Reconstitution assay system for ceramide transport with semi-intact cells
7. Visualizing mitochondrial lipids and fusion events in mammalian cells

8. Lipid Metabolism and Signaling:

9. Targeted and non-targeted analysis of membrane lipids using mass spectrometry
10. Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB
11. Acute Manipulation of Phosphoinositide Levels in Cells
12. Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins
13. Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach.
14. Devising powerful Genetics, Biochemical and Structural tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) across diverse species
15. Genome-wide screens for gene products regulating lipid droplet dynamics.

16. Imaging:

17. The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy
18. Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries
19. Analysis of cholesterol trafficking with fluorescent probes
20. Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes
21. Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes
22. Genetically encoded probes for phosphatidic acid