RNA Methodologies: A Laboratory Guide for Isolation and Characterization Book

	Preface
Ch. 1	RNA and the Cellular Biochemistry Revisited	2
Ch. 2	Transcription and the Organization of Eukaryotic Gene Sequences	12
Ch. 3	Messenger RNA	21
Ch. 4	Resilient Ribonucleases	33
Ch. 5	RNA Isolation Strategies	47
Ch. 6	Isolation of Polyadenylated RNA	94
Ch. 7	Electrophoresis: Principles and Parameters	113
Ch. 8	Electrophoresis of RNA	126
Ch. 9	The Northern Blot	159
Ch. 10	Nucleic Acid Probe Technology	175
Ch. 11	Practical Nucleic Acid Hybridization	192
Ch. 12	Principles of Detection	205
Ch. 13	Quantitation of Specific Messenger RNAs by the S1 Nuclease Assay	221
Ch. 14	Analysis of Nuclear RNA	236
Ch. 15	An RNA Paradigm	266
	Epilogue: A Few Pearls of Wisdom	271
	Appendix A A Deionization of Glyoxal	275
	Appendix B Phenol Preparation	277
	Appendix C Deoxyribonuclease I Incubation to Remove DNA from an RNA Sample	281
	Appendix D RNase Incubation to Remove RNA from a DNA Sample	283
	Appendix E Silanizing Centrifuge Tubes and Glassware	285
	Appendix F Treatment of Ethidium Bromide Solutions	287
	Appendix G Trypsinization Protocol	289
	Appendix H Isolation of High Molecular Weight DNA by the Salting-Out Procedure	291
	Appendix I Agarose Gel Electrophoresis with Methylmercuric Hydroxide	294
	Appendix J Polyacrylamide Gel Electrophoresis of RNA	297
	Appendix K Major Suppliers of Equipment and Reagents	300
	Glossary	304
	Index	311