Heterotrimeric G-Protein Effectors, Vol. 238 Book

	Contributors to Volume 238
	Preface
	Volumes in Series
1	Purification of Bovine Retinal cGMP Phosphodiesterase	3
2	Specific Peptide Probes for G-Protein Interaction with Effectors	13
3	Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures	31
4	Preparation and Use of "P"-Site-Targeted Affinity Ligands for Adenylyl Cyclases	56
5	Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo	71
6	G[subscript i] Assays in Transfected Cells	81
7	Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells	95
8	Transient Expression Assays for Mammalian Adenylyl Cyclases	108
9	Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers	116
10	Assays for G-Protein Regulation of Phospholipase C Activity	131
11	Chloride Current Assay for Phospholipase C in Xenopus Oocytes	140
12	Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-[beta] Isozymes	154
13	Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-[beta] Isozymes	168
14	Stimulation of Phospholipase C by G-Protein [beta] [gamma] Subunits	181
15	Purification of G-Protein-Regulated Phospholipase C from Turkey Erythrocytes	195
16	Phospholipase C Activity in Dictyostelium discoideum Using Endogenous Nonradioactive Phosphatidylinositol 4,5-Bisphosphate as Substrate	207
17	Purification of Phospholipase C-[beta]3 from Rat Brain	219
18	Characterization of Phospholipase C Isozymes in Bovine Retina: Purification of Phospholipase C-[beta]4	227
19	Purification of 110 kDa Phosphoinositide Phospholipase C Activated by G-Protein [beta] [gamma] Subunits	237
20	Amplification of Phosphatidylinositol-Specific Phospholipase C-[beta] Isoforms Using Degenerate Primers	244
21	Analysis of Guanine Nucleotides Associated with Protooncogene Ras	255
22	Measuring Activation of Kinases in Mitogen-Activated Protein Kinase Regulatory Network	259
23	Transcriptional Activation Analysis of Oncogene Function	271
24	Biological Assays for Cellular Transformation	277
25	Monitoring of Receptor-Mediated Changes in Intracellular Calcium at the Cellular and Subcellular Levels by Microfluorimetry and Imaging	297
26	Combination of Microfluorimetric Monitoring of Cytosolic Calcium and pH with Patch Clamp Electrophysiological Recordings in Neutrophil Granulocytes	308
27	G-Protein-Mediated Pathways Assayed by Electrophysiology and Confocal Microscopy	321
28	Purification and Reconstitution of N-Type Calcium Channel Complex from Rabbit Brain	335
29	Infusion of Guanine Nucleotides through Recording Electrodes for Studies on G-Protein Regulation of Ion Currents and Channels	348
30	Injection of Antisera into Cells to Study G-Protein Regulation of Channel Function	357
31	Whole-Cell Clamp Analysis for G-Protein Regulation of Channels	365
32	Whole-Cell Patch Recording in Brain Slices	375
33	Reconstitution of Receptor-Regulated Ion Channels in Isolated Patch Membrane	385
34	Regulation of Potassium Channels by G-Protein Subunits and Arachidonic Acid Metabolites	394
	Author Index	423
	Subject Index	443