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Preface v
Contributors xi
Sample Preparation Basics
Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization Stanley Goldberg 3
Bacteria and Yeast Cell Disruption Using Lytic Enzymes Oriana Salazar 23
Sample Solublization Buffers for Two-Dimensional Electrophoresis Walter Weiss Angelika Gorg 35
Quantitation of Protein in Samples Prepared for 2-D Electrophoresis Tom Berkelman 43
Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis Tom Berkelman 51
Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction Ulrich Schneider 63
Protein Labeling Techniques
Difference Gel Electrophoresis Based on Lys/Cys Tagging Reiner Westermeier Burghardt Scheibe 73
Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine Pier Giorgio Righetti Roberto Sebastiano Attilio Citterio 87
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Albrecht Gruhler Irina Kratchmarova 101
ICPL-Isotope-Coded Protein Label Josef Kellermann 113
Radiolabeling for Two-Dimensional Gel Analysis Helian Boucherie Aurelie Massoni Christelle Monribot-Espagne 125
Fractionation of Proteins by Chemical Reagents and Chromatography
Sequential Extraction of Proteins by Chemical Reagents Stuart J. Cordwell 139
Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg Gert Van den Bergh Lutgarde Arckens 147
Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis Ning Liu Aran Paulus 157
Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns Phillip Cash Ian R. Booth 167
Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures Hong Shen Xiang Li Charles J. Bieberich Douglas D. Frey 187
Fractionation of Proteins by Immobilized Metal Affinity Chromatography Xuesong Sun Jen-Fu Chiu Qing-Yu He 205
Fractionation of Proteins by Heparin Chromatography Sheng Xiong Ling Zhang Qing-Yu He 213
Fractionation of Proteins by Electrophoresis Methods
Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric Focusing Julie Hey Anton Posch Andrew Cohen Ning Liu Adrianna Harbers 225
Microscale Isoelectric Focusing in Solution Mee-Jung Han David W. Speicher 241
Prefractionation, Enrichment, Desalting and Depleting of Low Volume and Low Abundance Proteins and Peptides Using the MF10 Valerie Wasinger Linda Ly Anna Fitzgerald Brad Walsh 257
Sample Prefractionation in Granulated Sephadex IEF Gels Angelika Gorg Carsten Luck Walter Weiss 277
Free-Flow Electrophoresis System for Plasma Proteomic Applications Robert Wildgruber Jizu Yi Mikkel Nissum Christoph Eckerskorn Gerhard Weber 287
Protein Fractionation by Preparative Electrophoresis Michael Fountoulakis Ploumisti Dimitraki 301
Enrichment Strategies for Organelles, Multiprotein Complexes, and Specific Protein Classes
Isolation of Endocitic Organelles by Density Gradient Centrifugation Mariana Eca Guimaraes de Araujo Lukas Alfons Huber Taras Stasyk 317
Isolation of Highly Pure Rat Liver Mitochondria with the Aid of Zone-Electrophoresis in a Free Flow Device (ZE-FFE) Hans Zischka Josef Lichtmannegger Nora Jaegemann Luise Jennen Daniela Hamoller Evamaria Huber Axel Walch Karl H. Summer Martin Gottlicher 333
Isolation of Proteins and Protein Complexes by Immunoprecipitation Barbara Kaboord Maria Perr 349
Isolation of Phosphoproteins Lawrence G. Puente Lynn A. Megeney 365
Glycoprotein Enrichment Through Lectin Affinity Techniques Yehia Mechref Milan Madera Milos V. Novotny 373
Isolation of Bacterial Cell Membranes Proteins Using Carbonate Extraction Mark P. Molloy 397
Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems Henrik Everberg Niklas Gustavsson Folke Tjerneld 403
The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis Ki-Bum Kim Jae-Seon Lee Young-Gyu Ko 413
Isolation of Membrane Protein Complexes by Blue Native Electrophoresis Veronika Reisinger Lutz A. Eichacker 423
Tissue Microdissection Heidi S. Erickson John W. Gillespie Michael R. Emmert-Buck 433
Index 449
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Amber Tissandier
reviewed 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology) on July 13, 2020
2D PAGE: Sample Preparation and Fractionation: Volume 2
I listened to this short audiobook, which contrary to the "unabridged" description, is much much shorter than the book by the same title and author. It starts up with an in-depth look at how perception alters the response of cells. It discusses epigenetics, and how proteins really contain the "secret of life", and not DNA itself. So far, so good.
The author then tries to make a parallel between cellular perception and an organism's sense of perception. He describes ways in which perception and beliefs can alter one's biological functions. For example, stress can impair the immune system. And, the placebo effect has a strong impact on the defense against many diseases. This is true enough, but the book does not explain, at least to my satisfaction, how beliefs can have these effects. We learn the "what" but not the "how". Then, when the book brings in teachings of Jesus, I lost all hope for this book.
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Add 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology), The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of s, 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology) to the inventory that you are selling on WonderClubX
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Add 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology), The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of s, 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology) to your collection on WonderClub |