The Marvel Team-Up comic book series debuted with a March 1972 cover dated issue featuring Spider-Man and the Human Torch in a story by writer Roy Thomas and artist Ross Andru. The main artists on the series for the first several years were Andru, Gil Kane, Sal Buscema, and Jim Mooney. Spider-Man met the X-Men in issue #4, which was during a period when the mutant team's own title was featuring reprinted material.
The series often featured Spider-Man meeting characters who were not superheroes such as Werewolf by Night in issue #12 and the Frankenstein's Monster in issue #36. A multi-issue time travel story arc began in issue #41 with Spider-Man and the Scarlet Witch traveling to the Salem witch trials in 1692. The latter chapters of the story would include the Vision, Doctor Doom, and Moondragon. With issue #47, the series had a crossover with Marvel Two-in-One #17, which featured the Thing. Jean DeWolff was introduced as a supporting character in the Spider-Man/Iron Man story in issue #48.
John Byrne, who would later become the artist on The Uncanny X-Men, first drew the characters in Marvel Team-Up #53. Byrne and his Uncanny X-Men collaborator, writer Chris Claremont worked together on several issues of Marvel Team-Up. Captain Britain, a character created for Marvel UK, made his first appearance in an American comic book in Marvel Team-Up #65 (January 1978). The cast of NBC's Saturday Night Live appeared in issue #74 (October 1978), while issue #79 (March 1979) featured the spirit of Red Sonja possessing the body of Mary Jane Watson to face Kulan Gath.
Karma, a character that later joined the New Mutants, was created by writer Chris Claremont and artist Frank Miller in #100's lead story. A photo cover by Eliot R. Brown was used for the Spider-Man/Captain America team-up in issue #128. Marvel Team-Up #137 (January 1984) was part of the "Assistant Editors Month" event and featured (Aunt May and Franklin Richards battling Galactus. The series ended with issue #150 (February 1985) starring Spider-Man and the X-Men facing off with the Juggernaut and Black Tom Cassidy.
A Hulk and the Human Torch story written by Jack C. Harris and drawn by Steve Ditko in the 1980s that was intended for Marvel Team-Up was published by Marvel as Incredible Hulk and the Human Torch: From the Marvel Vault #1 in August 2011.
Spider-Man Team-Up was a brief attempt to revive the concept of the series and was soon followed by Marvel Team-Up vol. 2 which was published from September 1997 to July 1998. The third Marvel Team-Up series launched in January 2005 and ran for 25 issues which starred a variety of characters.