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FOREWORD ix
PREFACE xiii
ABBREVIATIONS xvii
1 INTRODUCTION OF SYSTEMS BIOLOGY IN TRADITIONAL CHINESE MEDICINE (TCM) 1
1.1 Characteristics and Compatibility Principles of Traditional Chinese Medicine (TCM) 1
1.2 Key Scientifi c Issues in TCM Modernization 9
1.3 Development of Systems Biology 16
1.4 Chemomics Integrated Systems Biology 19
1.5 Research Strategy and Prospective of Systems Biology in TCM 25
References 34
2 CHEMOMICS OF TRADITIONAL CHINESE MEDICINE 38
2.1 Characteristics and Research Diffi culties in TCM 39
2.2 Background of TCM Chemomics’ Proposal and Development 45
2.3 Chemomics 52
2.4 Chemomics and the Research of Formulas 58
References 63
3 TECHNOLOGICAL PLATFORM OF TCM CHEMOMICS 66
3.1 Acquisition Methods and Techniques for TCM Chemomics 66
3.2 Characterization Techniques of Chemome-TCM Fingerprinting 73
3.3 Information Processing for the Study of TCM Chemomics 107
3.4 Development of an Intelligent Quality Control System in the Process of Chinese Medicine Production 119
References 127
4 PHARMACOKINETIC INVESTIGATION ON TCM FORMULAS BASED ON GLOBAL SYSTEMS BIOLOGY 131
4.1 Pharmacokinetic Characteristics of TCM Formulas 131
4.2 Methodology of Pharmacokinetics of TCM Formulas 133
4.3 Application of PK-PD Model in the Toxicological Research of Liushen Pills (LSP) 138
4.4 Prospect 143
References 144
5 APPLICATION OF GENOMICS IN THE RESEARCH OF TCM 146
5.1 Genomics and TCM System Research 146
5.2 Prospect of Genomics in TCM Research 148
5.3 Cases of the Application of Genomics in TCM Research 148
References 168
6 PROTEOMICS STUDY OF TCM 171
6.1 Proteomics in TCM Research 171
6.2 A Case Study of Proteomics in TCM 174
6.3 The Application of High Content Screening in TCM Research 182
6.4 Limitations and Prospect of TCM Proteomics 186
References 187
7 APPLICATION OF METABONOMICS IN RESEARCH ON TCM 191
7.1 Current Research Situation of Metabonomics 191
7.2 Integration of Quantitative Metabonomics Platform Technology (QMPT) 191
7.3 Application of Metabonomics in the Field of Medicine 194
7.4 Examples of Metabonomic Research on TCM 198
References 206
8 APPLICATION OF CHEMOMETRICS AND BIOINFORMATICS IN TCM RESEARCH 210
8.1 Introduction of Chemometrics 210
8.2 Chemometric Techniques and Their Applications in TCM Research 211
8.3 Introduction of Bioinformatics 216
8.4 Bioinformatics Techniques and Their Applications in the Research of TCM 219
8.5 Conclusions 222
References 224
9 STUDY OF INTEGRATED BIOMARKER SYSTEM OF DIABETIC NEPHROPATHY 228
9.1 Introduction of Diabetic Nephropathy 228
9.2 Mogensen Staging and TCM Typing of DN 234
9.3 The Metabonomics Study of DN 244
9.4 Condition of Metabolism after Treatment with TangShen Formula (TSF) 278
9.5 Genomics Study of Diabetic Neuropathy 286
9.6 The Integrated Biomarker System of DN 301
9.7 Conclusions 311
References 312
10 CHEMOMICS RESEARCH ON THE TCM FORMULA OF THE QINGKAILING INJECTION 317
10.1 Chemomics Research of Qingkailing Injection 318
10.2 Pharmacodynamic Evaluation of Qingkailing Injection 330
10.3 Research on the Qingkailing Derived Formula 335
10.4 Conclusions 368
References 369
11 INTEGRATED GLOBAL SYSTEMS BIOLOGY FOR THE RESEARCH AND DEVELOPMENT OF CHINESE MEDICINE SHUANGLONG FORMULA 371
11.1 Brief Introduction to the Shuanglong Formula 371
11.2 Chemomics Study of the Shuanglong Formula 374
11.3 Pharmacodynamic Evaluation of SLF Effective Ingredients 384
11.4 Systems Biology Study of the Mechanism of Directed Differentiation of Stem Cells Induced by NSLF6 402
11.5 Anti-Myocardial Infarction Effect of NSLF6 426
11.6 Conclusions 429
References 430
12 DEMONSTRATIVE RESEARCH ON THE SAFETY EVALUATION OF LIUSHEN PILLS 437
12.1 Introduction 437
12.2 Toxicity Study of Chansu 438
12.3 Chemomics Study of LSP 446
12.4 Assessment of Effectiveness and Safety of LSP 457
12.5 In Vivo Distribution and Metabonomics of LSP and Xionghuang 462
12.6 Metabonomic Research of LSP 467
12.7 Conclusions 477
References 478
INDEX 481
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