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Preface.
Chapter 1. Introduction to Bioseparations.
a. Statement of the problem.
b. Engineering Developments.
c. Current approaches to bioseparations.
Chapter 2. Thermodynamics.
a. A brief introduction.
b. Thermodynamics of open systems.
c. Thermodynamic analysis of processes.
Chapter 3. Principles of Mass Transfer.
1. Diffusion.
2. Fluxes.
3. Law of conservation of mass.
4. Examples.
Chapter 4. General Properties of Bioproducts.
1. Proteins.
a. Structure.
b. Stability (solubility and aggregation).
c. Separation of complex protein mixtures by two-dimensional electrophoresis.
2. Nucleic Acids.
a. Structure.
b. Properties.
3. Laboratory experiments.
Chapter 5. Cell disruption.
1. General approach to cell disruption.
a. Types of cells.
b. Location of product.
c. Physical Chemistry Properties of Cells (as they relate to disruption).
2. Bead Mills.
3. Homogenizers.
4. Chemical Methods.
5. Biochemical Methods.
6. Laboratory experiments.
Chapter 6. Separation of Solids.
1. General Principles.
a. Flocculation of proteins.
b. Inclusion bodies.
2. Filtration.
a. Conventional Filtration.
b. Ultrafiltration.
3. Centrifugation.
4. Sedimentation.
5. Laboratory experiments.
6. Liquid/liquid extraction.
Chapter 7. Chromatography.
1. General Principles.
2. Gel permeation.
3. Ion Exchange Chromatography.
4. Hydrophobic Interactions Chromatography.
5. Affinity Chromatography.
6. Simulated Moving Bed Chromatography.
7. Laboratory Experiments.
Chapter 8. Protein Precipitation.
1. Precipitation by salting-out.
2. Isoelectric Precipitation.
3. Precipitation by non-ionic polymers.
4. LaboratoryExperiments.
Chapter 9. Polishing.
1. Freeze drying.
2. Crystallization.
3. Product Formulation.
4. Laboratory Experiments.
Chapter 10. Separations in a Chip.
1. Introduction to micro-devices.
2. Fluidics.
3. Micro-reactors, micro-mixers, and valves.
4. Chromatography and electrophoresis in a chip.
5. Micro-sensors.
Chapter 11. Design of Separation cascades.
1. General Principles.
2. Examples.
3. Laboratory Projects.
Appendix. Experimental Design.
1. Introduction to Experimental Design.
2. Precision and Accuracy.
3. Descriptive Statistics.
4. Statistical Tests.
5. Examples.
Table of Contents.
Chapter 1. Introduction to Bioseparations.
a. Statement of the problem.
b. Engineering Developments.
c. Current approaches to bioseparations.
Chapter 2. Thermodynamics.
a. A brief introduction.
b. Thermodynamics of open systems.
c. Thermodynamic analysis of processes.
Chapter 3. Principles of Mass Transfer.
1. Diffusion.
2. Fluxes.
3. Law of conservation of mass.
4. Examples.
Chapter 4. General Properties of Bioproducts.
1. Proteins.
a. Structure.
b. Stability (solubility and aggregation).
c. Separation of complex protein mixtures by two-dimensional electrophoresis.
2. Nucleic Acids.
a. Structure.
b. Properties.
3. Laboratory experiments.
Chapter 5. Cell disruption.
1. General approach to cell disruption.
a. Types of cells.
b. Location of product.
c. Physical Chemistry Properties of Cells (as they relate to disruption).
2. Bead Mills.
3. Homogenizers.
4. Chemical Methods.
5. Biochemical Methods.
6. Laboratory experiments.
Chapter 6. Separation of Solids.
1. General Principles.
a. Flocculation of proteins.
b. Inclusion bodies.
2. Filtration.
a. Conventional Filtration.
b. Ultrafiltration.
3. Centrifugation.
4. Sedimentation.
5. Laboratory experiments.
6. Liquid/liquid extraction.
Chapter 7. Chromatography.
1. General Principles.
2. Gel permeation.
3. Ion Exchange Chromatography.
4. Hydrophobic Interactions Chromatography.
5. Affinity Chromatography.
6. Simulated Moving Bed Chromatography.
7. Laboratory Experiments.
Chapter 8. Protein Precipitation.
1. Precipitation by salting-out.
2. Isoelectric Precipitation.
3. Precipitation by non-ionic polymers.
4. Laboratory Experiments.
Chapter 9. Polishing.
1. Freeze drying.
2. Crystallization.
3. Product Formulation.
4. Laboratory Experiments.
Chapter 10. Separations in a Chip.
1. Introduction to micro-devices.
2. Fluidics.
3. Micro-reactors, micro-mixers, and valves.
4. Chromatography and electrophoresis in a chip.
5. Micro-sensors.
Chapter 11. Design of Separation cascades.
1. General Principles.
2. Examples.
3. Laboratory Projects.
Appendix. Experimental Design.
1. Introduction to Experimental Design.
2. Precision and Accuracy.
3. Descriptive Statistics.
4. Statistical Tests.
5. Examples.
Index
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Mary Maginley
reviewed Industrial Bioseparations: Principles and Practice on October 06, 2019
Industrial Bioseparations: Principles and Practice
I picked this up and put it down again a few times, but I'm so glad I stuck with it. Very thoughtful and worthwhile ethnography discussing the Bhopal disaster from many perspectives. The book tackles not just the disaster but the many reactions to it: across time (immediately after and in the years and decades that follow), across communities (in India and the U.S), and from many perspectives.
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Add Industrial Bioseparations: Principles and Practice, Industrial Bioseparations offers comprehensive coverage of bioseparations including all unit operations. This new book offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today. It i, Industrial Bioseparations: Principles and Practice to the inventory that you are selling on WonderClubX
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Add Industrial Bioseparations: Principles and Practice, Industrial Bioseparations offers comprehensive coverage of bioseparations including all unit operations. This new book offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today. It i, Industrial Bioseparations: Principles and Practice to your collection on WonderClub |