Genetic Recombination: Reviews and Protocols Book

Part I. Studying Recombination Events in Eukaryotes

Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae

Rachelle Miller Spell and Sue Jinks-Robertson

Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells

Jason A. Smith and Alan S. Waldman

Intrachromosomal Homologous Recombination in Arabidopsis thaliana

Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta

Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease

Jac A. Nickoloff and Mark A. Brenneman

Transformation of Monomorphic and Pleomorphic Trypanosoma brucei

Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry

Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice

Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima

Part II. Recombination as a Reporter of Genomic Instability

Detecting Carcinogens With the Yeast DEL Assay

Richard J. Brennan and Robert H. Schiestl

In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer

Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl

Part III. Recombination as a Tool for Producing Targeted Genetic Modification

Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms

Mark D. Baker

DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations

Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis

Targeted Gene Modification Using Triplex-Forming Oligonucleotides

Jean Y. Kuan and Peter M. Glazer

Using Nucleases to Stimulate Homologous Recombination

Dana Carroll

Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA

Katie K. Maguire and Eric B. Kmiec

Part IV. Biochemistry of Recombination

Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination

Tamara Goldfarb and Eric Alani

Holliday Junction Branch Migration and Resolution Assays

Angelos Constantinou and Stephen C. West

Index