Fibrosis Research: Methods and Protocols Book

Part I. The Clinical Scope of Fibrosis

Pulmonary Fibrosis

David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, and Joseph P. Lynch, III

Renal Fibrosis

H. William Schnaper

Transforming Growth Factor-b: A Key Mediator of Fibrosis

Alain Mauviel

Part II. Regulation of Extracellular Matrix Metabolism: Cultured Cells

Isolation and Culture of Skin Fibroblasts

Laure Rittié and Gary J. Fisher

Isolation and Culture of Hepatic Stellate Cells

Ralf Weiskirchen and Axel M. Gressner

Isolation and Phenotypic Characterization of Lung Fibroblasts

Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith, and Richard P. Phipps

Methods for Measuring Type I Collagen Synthesis In Vitro

David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, and Ronald H. Goldstein

Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation

Richard A. Rippe and Branko Stefanovic

Methods for Measuring TGF-b Using Antibodies, Cells, and Mice

Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, and Daniel B. Rifkin

Part III. Studying Fibrosis Using Animal Models

Morphological Methods for Assessment of Fibrosis

Rakesh K. Kumar

Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation

Robin J. McAnulty

Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens

Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, and Steven L. Kunkel

Animal Models for Adult Dermal Wound Healing

Mary Birch, Annette Tomlinson, and Mark W. J. Ferguson

Modeling Liver Fibrosis in Rodents

Christothea Constandinou, Neil Henderson, and John P. Iredale

Animal Models of Pulmonary Fibrosis

Mehrnaz Gharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan

Animal Models of Renal Fibrosis

Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri

Animal Models of Cardiac Fibrosis

Yao Sun and Karl T. Weber

Part IV. Genetic Approaches to Studying Fibrosis

Finding Fibrosis Genes: The Lung

Lauranell H. Burch and David A. Schwartz

Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations

Christopher Paul Day

Analysis of Microarray Experiments for Pulmonary Fibrosis

Nilesh B. Davé and Naftali Kaminski

DNA Microarrays and Data Mining to Study Hepatic Fibrosis

Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, and Ramón Bataller

Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype: The Molecular Approach for Liver Fibrosis

Norifumi Kawada

Index