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Biotechnology Book

Biotechnology
Biotechnology, This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein , Biotechnology has a rating of 3 stars
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Biotechnology, This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein , Biotechnology
3 out of 5 stars based on 2 reviews
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  • Biotechnology
  • Written by author Teresa Thiel,Shirley T. Bissen,Eilene M. Lyons
  • Published by New York, NY : McGraw-Hill, c2002., 2001/09/01
  • This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein
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Examine Enzyme Activity of alpha-Amylase1 Starch Plate Assay 2 Quantitative Enzyme Assay 3 Factors Affecting Enzyme Function Examine alpha-Amylase Proteins 4 Analysis of Protein Structure Using RasMol 5 Analysis of alpha-Amylase Proteins 5A SDS-PAGE 5B Western Blotting Examine DNA Structure 6 Analysis of DNA Structure Using RasMol 7 Isolation of Chromosomal DNA from Bacillus licheniformis Find the alpha-Amylase Gene 8 PCR Amplification and Labeling of Probe DNA 9 Southern Hybridization 9A Restriction Enzyme Cleavage of Chromosomal DNA 9B Denaturation and Transfer of DNA to a Membrane 9C Southern Hybridization and Detection Clone the alpha-Amylase Gene 10 Cloning the alpha-Amylase Gene 10A Cleavage of Chromosomal DNA 10B Cleavage of Plasmid DNA 10C Ligation of Chromosomal and Plasmid DNA 10D Transformation 10E Identification of alpha-Amylase Clones Analyze alpha-Amylase Clones 11 Verification and Mapping of alpha-Amylase Clones (and more...)


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