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Abbreviations | ||
Foreword | 1 | |
Introduction | 1 | |
Pt. 1 | Fundamental mechanisms in immunology | 3 |
1.1 | Non-specific defense mechanisms: natural immunity | 3 |
1.2 | Antigens and antibodies | 5 |
1.3 | Cells involved in immune responses | 7 |
1.4 | Lymphoid organs | 17 |
1.5 | Peripheral circulation of lymphocytes from lymphoid organs | 27 |
1.6 | Transformation and interaction of lymphocytes during active immune phenomena | 29 |
1.7 | The role of macrophages and monocytes, their equivalent in blood - Antigen presentation | 35 |
1.8 | Soluble cellular mediators or cytokines | 41 |
1.9 | Immunoglobulins | 45 |
1.10 | Monoclonal antibodies (Mab) - Chimeric antibodies and repertoire cloning | 55 |
1.11 | Complement | 71 |
1.12 | The four basic types of immuno-allergic reactions | 75 |
1.13 | Immunological tolerance | 93 |
Pt. 2 | Common allergic diseases | 99 |
2.1 | Atopy: a genetic predisposition | 99 |
2.2 | Periodical or seasonal rhinitis: hay fever | 100 |
2.3 | Perennial or non-seasonal rhinitis | 101 |
2.4 | Asthma | 101 |
2.5 | Urticaria and angioneurotic edema | 108 |
2.6 | Contact dermatitis | 108 |
2.7 | Atopic dermatitis (or atopic eczema) | 109 |
2.8 | Farmer's lung and other type III immune complex diseases (extrinsic alveolitis) | 109 |
2.9 | Skin tests in allergy | 111 |
2.10 | Hyposensitization - immunotherapy | 112 |
2.11 | Outline of therapy for type I allergies | 113 |
Pt. 3 | Elements of immune pathology | 114 |
3.1 | Immunoglobulin diseases | 114 |
3.2 | Immune deficiencies | 114 |
3.3 | Acquired immunodeficiency syndrome: AIDS | 117 |
3.4 | Renal immunopathology | 123 |
3.5 | Organ transplantation: rejection phenomenon | 124 |
3.6 | Graft versus host reaction (GVH) | 129 |
3.7 | Immunosuppressive therapies | 131 |
3.8 | Autoimmune diseases | 133 |
Pt. 4 | Allergens and vaccines | 137 |
4.1 | Native and recombinant allergens | 137 |
4.2 | Antitoxin, antimicrobial and antiviral vaccines | 139 |
4.3 | Malaria: the search for a vaccine. The difficulties | 141 |
4.4 | Research and hopes in immune therapy of cancer | 143 |
4.5 | Vaccination of fish | 149 |
Pt. 5 | Immunological tests in vitro | 151 |
5.1 | Immunodiffusion | 151 |
5.2 | Determination of the main protein fractions in serum by radial immunodiffusion (Mancini's technique) | 153 |
5.3 | Protein electrophoresis | 155 |
5.4 | Immunoelectrophoresis | 157 |
5.5 | Agglutination and passive hemagglutination | 159 |
5.6 | Immunofluorescence | 161 |
5.7 | Chemoluminescence | 165 |
5.8 | Enzyme-linked immunosorbent assay (ELISA) | 165 |
5.9 | Immunoprint (Western blot) | 167 |
5.10 | Determination of allergen-specific IgE: RAST/CAP | 169 |
5.11 | Other techniques for IgE determination | 171 |
5.12 | Rosette tests | 175 |
5.13 | Lymphocyte proliferation test | 177 |
5.14 | Flow cytometry (FACS) | 179 |
5.15 | Release tests for cellular mediators | 181 |
5.16 | Molecular biology, a twin sister of immunology. A quick test for detection of tuberculosis | 183 |
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