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Preface vii
Contributors xi
Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Cecilia A. Roque Christopher R. Lowe 1
Various Modes of Affinity Chromatography
Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton Michael Zachariou 25
Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar Igor Yu. Galaev Bo Mattiasson 37
Immunoaffinity Chromatography Stuart R. Gallant Vish Koppaka Nick Zecherle 53
Dye Ligand Chromatography Stuart R. Gallant Vish Koppaka Nick Zecherle 61
Purification of Proteins Using Displacement Chromatography Nihal Tugcu 71
Affinity Chromatography Using Purification Tags
Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein L Ana Cecilia A. Roque Christopher R. Lowe 93
Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography Joanne L. Casey Andrew M. Coley Michael Foley 111
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 125
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins Adam Charlton Michael Zachariou 137
Methods for the Purification of HQ-Tagged Proteins Becky Godat Laurie Engel Natalie A. Betz Tonny M. Johnson 151
Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding Methods Leonard K. Pattenden Walter G. Thomas 169
Methods for Detection of Protein-Protein and Protein-DNA Interactions Using HaloTag Marjeta Urh Danette Hartzell Jacqui Mendez Dieter H. Klaubert Keith Wood 191
Site-Specific Cleavage of Fusion Proteins Adam Charlton 211
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags Jose Arnau Conni Lauritzen Gitte Ebert Petersen John Pedersen 229
Various Applications of Affinity Chromatography
Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev Bo Mattiasson 247
Monolithic Bioreactors for Macromolecules Mojca Bencina Katja Bencina Ales Podgornik Ales Strancar 257
Plasmid DNA Purification Via the Use of a Dual Affinity Protein Gareth M. Forde 275
Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 285
Protein Separation Using Immobilized Phospholipid Chromatography Tzong-Hsien Lee Marie-Isabel Aguilar 295
Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Niels H. H. Heegaard Christian Schou Jesper Ostergaard 303
Index 339
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Add Affinity Chromatography: Methods and Protocols, Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods , Affinity Chromatography: Methods and Protocols to the inventory that you are selling on WonderClubX
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Add Affinity Chromatography: Methods and Protocols, Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods , Affinity Chromatography: Methods and Protocols to your collection on WonderClub |