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Preface v
Contributors ix
1 Introduction to Microarray Technology Martin Dufva 1
2 Probe Design for Expression Arrays Using OligoWiz Rasmus Wernersson 23
3 Comparative Genomic Hybridization: Microarray Design and Data Interpretation Richard Redon and Nigel P. Carter 37
4 Design of Tag SNP Whole Genome Genotyping Arrays Daniel A. Peiffer
5 Fabrication of DNA Microarray Martin Dufva 63
6 Immobilization Chemistries Sascha Todt
7 Fabrication Using Contact Spotter Annelie Walden Peter Nilsson 101
8 RNA Preparation and Characterization for Gene Expression Studies Michael Stangegaard 115
9 Gene Expression Analysis Using Agilent DNA Microarrays Michael Stangegaard 133
10 Target Preparation for Genotyping Specific Genes or Gene Segments Jesper Petersen Lena Poulsen Martin Dufva 147
11 Genotyping of Mutations in the Beta-Globin Gene Using Allele Specific Hybridization Lena Poulsen Jesper Petersen Martin Dufva 157
12 Microarray Temperature Optimization Using Hybridization Kinetics Steve Blair Layne Williams Justin Bishop Alexander Chagovetz 171
13 Whole-Genome Genotyping on Bead Arrays Kevin L. Gunderson 197
14 Genotyping Single Nucleotide Polymorphisms by Multiplex Minisequencing Using Tag-Arrays Lili Milani Ann-Christine Syvanen 215
15 Resequencing Arrays for Diagnostics of Respiratory Pathogens Baochuan Lin Anthony P. Malanoski 231
16 Comparative Genomic Hybridization: DNA Preparation for Microarray Fabrication Richard Redon Diane Rigler Nigel P. Carter 259
17 Comparative Genomic Hybridization: DNA Labeling, Hybridization and Detection Richard Redon Tomas Fitzgerald Nigel P. Carter 267
18 Chromatin Immunoprecipitation Using Microarrays MickaelDurand-Dubief Karl Ekwall 297
Index 297
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Tracy Franklin
reviewed DNA Microarrays for Biomedical Research: Methods and Protocols, Vol. 529 on September 01, 2014
DNA Microarrays for Biomedical Research
[
The good:
- bold theorizing! But testable and falsifiable, so it has more merit than technically savvy sci-fi. (still, all it changes is the mechanism. It doesn't address the selection pressures, why intelligence, or many of the objections he raises to a darwinian account)
- many important and fascinating experiments are described
- even if you don't buy his theory you will learn a lot of interesting facts about genetics and epigenetics, the technical level is high and, especially in the last couple of parts, very satisfying.
- Fills a niche, not a lot of interesting material on the subject that's available to the general reader.
The bad:
- didn't make it clear to me why his evolutionary stories were more plausible and useful than all the ones he dismisses (even tho I find his exercises in making alternate accounts pedagogically useful)
- Apparently the discovery of fire and heat-preparation of food was not an event worth mentioning, even tho this is interpreted as causing the shortening of our intestines which lead to much better energy utilization (the intestines need a lot of energy to do their work), freeing up resources for a power-hungry organ like the brain
- although he did make a point of the importance of brain plasticity, he never really addresses the long developmental time of human children and the extent of dendritic pruning that takes place during this period. Having a longer window for critical and sensitive periods sounds like an important factor in how much culture can change an organism.
- his discussion of how traits like intelligence or musicality (or indeed a brain region specific to written language) was useless to our pre-historic forefathers wasn't entirely convincing (what good is a cubic-root to a caveman? I'm not sure, but pattern recognition and being able to think and manipulate abstractions in some capacity sounds like it could be useful? It does sound like it's a subject that has been covered by others, so I should perhaps not hold it against him for not dedicating more time to the subject). (hide spoiler)]
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Add DNA Microarrays for Biomedical Research: Methods and Protocols, Vol. 529, DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DN, DNA Microarrays for Biomedical Research: Methods and Protocols, Vol. 529 to the inventory that you are selling on WonderClubX
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Add DNA Microarrays for Biomedical Research: Methods and Protocols, Vol. 529, DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DN, DNA Microarrays for Biomedical Research: Methods and Protocols, Vol. 529 to your collection on WonderClub |