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Preface
Contributors
Part I: Introduction
1. Transgenic Wheat, Barley and Oats: Production and Characterization
Paul A. Lazzeri and Huw D. Jones
Part II: Transformation and Regeneration
2. Selection of Transformed Plants
Huw D. Jones and Caroline A. Sparks
3. Reporter Genes
Alison Huttly
4. Biolistics Transformation of Wheat
Caroline A. Sparks and Huw D. Jones
5. Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos
Huixia Wu, Angela Doherty and Huw D. Jones
6. Floral Transformation of Wheat
Sujata Agarwal, Star Loar, Camille Steber and Janice Zale
7. Highly Efficient Agrobacterium-Mediated Transformation of Wheat via In Planta Inoculation
Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul and Tina Barsby
8. Barley Transformation Using Biolistic Techniques
Wendy A. Harwood and Mark A. Smedley
9. Barley Transformation Using Agrobacterium-Mediated Techniques
Wendy A. Harwood, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry, Mark A. Smedley, Nicola Leyland and John W. Snape
10. Transformation of Oats and its Application to Improving Osmotic Stress Tolerance
Shahina B. Maqbool, Heng Zhong, Hesham F. Oraby and Mariam B. Sticklen
Part III: Gene and Protein Expression
11. Promoter Sequences for Defining Transgene Expression
Huw D. Jones and Caroline A. Sparks
12. Down-Regulation of Gene Expression by RNA-Induced Gene Silencing
Silvia Travella and Beat Keller
Part IV: Characterisation of Transgenic Plants
13. Gene Insertion Patterns and Sites
Phillippe Vain and Vera Thole
14. Fluorescent In Situ Hybridization to Detect Transgene Integration Into Plant Genomes
Trude Schwartzacher
15. Establishing Substantial Equivalence: Transcriptomics
María Marcela Baudo, Stephen J. Powers, Rowan A.C. Mitchell and Peter R. Shewry
16. Establishing Substantial Equivalence: Proteomics
Alison Lovegrove, Louise Salt and Peter R. Shewry
17. Establishing Substantial Equivalence: Metabolomics
Michael H. Beale, Jane L. Ward and John M. Baker
18. Design and Management of Field Trials of Transgenic Cereals
Zoltán Bedo, Mariann Rakszegi and László Láng
19. GM Risk Assessment
Penelope A.C. Sparrow
Part V: Conclusions
20. Transgenic Wheat, Barley and Oats: Future Prospects
Jim M. Dunwell
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