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Medical BioMethods Handbook Book

Medical BioMethods Handbook
Medical BioMethods Handbook, , Medical BioMethods Handbook has a rating of 4.5 stars
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Medical BioMethods Handbook, , Medical BioMethods Handbook
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  • Medical BioMethods Handbook
  • Written by author John M. Walker
  • Published by Springer-Verlag New York, LLC, March 2005
  • The editors (both of the U. of Hertfordshire, UK) present chapters covering 44 of the most frequently used molecular and biochemical techniques in medical and clinical research, especially diagnostics. Contributors describe the theory behind each techniqu
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Book Categories

Authors

1Basic techniques in molecular biology1
2Probe design, production, and applications13
3Restriction enzymes : tools in clinical research25
4Southern blotting as a diagnostic method35
5Western blotting as a diagnostic method43
6Principles and medical applications of the polymerase chain reaction63
7Single-strand conformation polymorphism (SSCP) analysis73
8Denaturing gradient get electrophoresis (DGGE)79
9Quantitative analysis of DNA sequences by PCR and solid-phase sequencing87
10Introduction to capillary electrophoresis of DNA : biomedical applications95
11Mapping techniques117
12Dideoxyfingerprinting for mutation detection135
13Conformation-sensitive gel electrophoresis147
14Amplification refractory mutation system and molecular diagnostics155
15Ligase chain reaction167
16Chemical cleavage of mismatch : theory and clinical applications183
17The protein truncation test195
18Linkage, allele sharing, and association205
19Single-nucleotide polymorphisms : technology and applications227
20cDNA microarrays255
21Nucleic acid sequence-based amplification273
22Oligonucleotide ligation assay293
23Quantitative TaqMan real-time PCR : diagnostic and scientific applications305
24Use of denaturing high-performance liquid chromatography in molecular medicine315
25Quantitative PCR327
26Liquid chromatography-mass spectrometry of nucleic acids347
27Comparative genomic hybridization in clinical and medical research375
28Bioinformatics tools for gene and protein sequence analysis387
29In situ hybridization409
30Enzyme-linked immunosorbent assay419
31Protein therapeutics : mouse, humanized, and human antibodies429
32Karyotyping447
33Microsatellite analysis463
34Analysis of chromosomal translocations471
35Differential display : theory and applications487
36Techniques for gene expression profiling507
37Capillary electrophoresis in clinical analysis519
38Flow cytometry in the biomedical arena531
39Immunocytochemistry555
40Ribotyping in clinical microbiology573
41Diagnostic applications of protein microarrays583
42The human genome project593
43Prenatal diagnosis of inborn errors of metabolism607
44Gene therapy : methods and application625


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