Basic Cell Culture Protocols Book

1	Culture of primary adherent cells and a continuously growing nonadherent cell line	1
2	Detection of mycoplasma contaminations	13
3	Eradication of mycoplasma contaminations	25
4	Authentication of scientific human cell lines : easy-to-use DNA fingerprinting	35
5	Cytogenetic analysis of cell lines	51
6	Human and mouse hematopoietic colony-forming cell assays	71
7	Isolation and culture of murine macrophages	91
8	Isolation and culture of human macrophages	105
9	Development of T-lymphocytes in mouse fetal thymus organ culture	117
10	In vitro generation of lymphocytes from embryonic stem cells	135
11	Hematopoietic development of human embryonic stem cells in culture	149
12	Generation of murine stromal cell lines : models for the microenvironment of the embryonic mouse aorta-gonads-mesonephros region	163
13	Culture of human and mouse mesenchymal cells	173
14	Isolation, purification, and cultivation of murine and human keratinocytes	187
15	Isolation and culture of primary human hepatocytes	207
16	Primary kidney proximal tubule cells	231
17	Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity	249
18	Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue	265
19	Isolation and culture of skeletal muscle myofibers as a means to analyze-satellite cells	281
20	Adult ventricular cardiomyocytes : isolation and culture	305
21	Isolation and culture of primary endothelial cells	315
22	Studying leukocyte rolling and adhesion in vitro under flow conditions	331
23	Isolation and characterization of side population cells	343
24	Scalable production of embryonic stem cell-derived cells	353