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Recombinant Gene Expression Book

Recombinant Gene Expression
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  • Recombinant Gene Expression
  • Written by author Paulina Balbas
  • Published by Springer-Verlag New York, LLC, December 2009
  • For this second edition of a collection of research protocols for molecular biologists, Balbas (Centro de Investigacion en Biotecnologia, Mexico) and Lorence (Virginia Polytechnic Institute and State University) have added critical review articles that su
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Prefacev
Contributorsxiii
Part I.General Issues About Recombinant Gene Expression
1Host Cell Compatibility in Protein Expression3
2Production of Recombinant Proteins: Challenges and Solutions15
3Folding-Promoting Agents in Recombinant Protein Production53
Part II.Prokaryotes
4Back to Basics: pBR322 and Protein Expression Systems in E. coli77
5[alpha]-Complementation Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments91
6Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli101
7Overexpression of Chromosomal Genes in Escherichia coli113
8Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli123
9Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli135
10Copy-Control pBAC/oriV Vectors for Genomic Cloning145
11Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV155
12Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation169
13Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae183
14Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria209
15Recombinant Protein Production in Antarctic Gram-Negative Bacteria225
Part III.Fungi
16Recombinant Protein Production in Yeasts241
17Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae259
18High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae267
19High-Throughput Expression in Microplate Format in Pichia pastoris277
20Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae287
21Three Decades of Fungal Transformation: Key Concepts and Applications297
22Three Decades of Fungal Transformation: Novel Technologies315
Part IV.Plants and Plant Cells
23Gene Transfer and Expression in Plants329
24Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors351
25Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens365
26New Selection Marker for Plant Transformation385
27Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants397
Part V.Animals and Animal Cells
28Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals417
29Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines435
30Inducible Gene Expression in Mammalian Cells and Mice451
31Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells467
32Generation of High-Recombinant Protein-Producing Chinese Hamster Ovary (CHO) Cells477
33Preparation of Recombinant Proteins in Milk485
Index495


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