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Preface | v | |
Contributors | xiii | |
Part I. | General Issues About Recombinant Gene Expression | |
1 | Host Cell Compatibility in Protein Expression | 3 |
2 | Production of Recombinant Proteins: Challenges and Solutions | 15 |
3 | Folding-Promoting Agents in Recombinant Protein Production | 53 |
Part II. | Prokaryotes | |
4 | Back to Basics: pBR322 and Protein Expression Systems in E. coli | 77 |
5 | [alpha]-Complementation Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments | 91 |
6 | Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli | 101 |
7 | Overexpression of Chromosomal Genes in Escherichia coli | 113 |
8 | Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli | 123 |
9 | Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli | 135 |
10 | Copy-Control pBAC/oriV Vectors for Genomic Cloning | 145 |
11 | Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV | 155 |
12 | Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation | 169 |
13 | Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae | 183 |
14 | Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria | 209 |
15 | Recombinant Protein Production in Antarctic Gram-Negative Bacteria | 225 |
Part III. | Fungi | |
16 | Recombinant Protein Production in Yeasts | 241 |
17 | Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae | 259 |
18 | High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae | 267 |
19 | High-Throughput Expression in Microplate Format in Pichia pastoris | 277 |
20 | Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae | 287 |
21 | Three Decades of Fungal Transformation: Key Concepts and Applications | 297 |
22 | Three Decades of Fungal Transformation: Novel Technologies | 315 |
Part IV. | Plants and Plant Cells | |
23 | Gene Transfer and Expression in Plants | 329 |
24 | Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors | 351 |
25 | Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens | 365 |
26 | New Selection Marker for Plant Transformation | 385 |
27 | Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants | 397 |
Part V. | Animals and Animal Cells | |
28 | Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals | 417 |
29 | Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines | 435 |
30 | Inducible Gene Expression in Mammalian Cells and Mice | 451 |
31 | Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells | 467 |
32 | Generation of High-Recombinant Protein-Producing Chinese Hamster Ovary (CHO) Cells | 477 |
33 | Preparation of Recombinant Proteins in Milk | 485 |
Index | 495 |
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