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Chromosome Analysis Protocols Book

Chromosome Analysis Protocols
Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols has a rating of 3 stars
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Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols
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  • Chromosome Analysis Protocols
  • Written by author John R. Gosden
  • Published by Springer-Verlag New York, LLC, February 1994
  • Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe
  • Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Cha
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Preface
Contributors
Ch. 1Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution1
Ch. 2Chromosome Preparation from Hematological Malignancies11
Ch. 3Meiotic Chromosome Preparation27
Ch. 4Preparation of Chromosomes for Scanning Electron Microscopy41
Ch. 5Immortalized Cell Lines: Chromosome Preparation and Banding51
Ch. 6Chromosome Banding and Identification Absorption Staining59
Ch. 7Chromosome Banding and Identification: Fluorescence83
Ch. 8Chromosome Banding: Stain Combinations for Specific Regions97
Ch. 9Inhibition of Chromosome Condensation113
Ch. 10Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity123
Ch. 11Automatic Karyotype Analysis141
Ch. 12Bivariate Chromosome Analysis Using a Commercial Flow Cytometer187
Ch. 13Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping205
Ch. 14Electrophoretic Karyotype Analysis: Pulsed Field Gel Electrophoresis221
Ch. 15Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations253
Ch. 16Immunocytochemical Techniques Applied to Meiotic Chromosomes287
Ch. 17Immortalized Cell Lines: Their Creation and Use in Gene Mapping303
Ch. 18Oligonucleotide Primed in Situ DNA Synthesis (PRINS): An Alternative to in Situ Hybridization for Gene Mapping and the Inversion of Genome Organization323
Ch. 19Electron Microscopic Localization of in Situ Hybrids335
Ch. 20Protocols for Chromosome-Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping353
Ch. 21Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines379
Ch. 22Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints403
Ch. 23Chromosome Dissection and Cloning425
Ch. 24Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization437
Ch. 25Molecular Analysis of Chromosome Aberrations: In Situ Hybridization449
Ch. 26Alu- and L1-Primed PCR-Generated Probes for Nonisotopic in Situ Hybridization479
Ch. 27Chromosome Substructure Investigation: Telomeres493
Index505


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Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols

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Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols

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Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols

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