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Preface | ||
Contributors | ||
Ch. 1 | Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution | 1 |
Ch. 2 | Chromosome Preparation from Hematological Malignancies | 11 |
Ch. 3 | Meiotic Chromosome Preparation | 27 |
Ch. 4 | Preparation of Chromosomes for Scanning Electron Microscopy | 41 |
Ch. 5 | Immortalized Cell Lines: Chromosome Preparation and Banding | 51 |
Ch. 6 | Chromosome Banding and Identification Absorption Staining | 59 |
Ch. 7 | Chromosome Banding and Identification: Fluorescence | 83 |
Ch. 8 | Chromosome Banding: Stain Combinations for Specific Regions | 97 |
Ch. 9 | Inhibition of Chromosome Condensation | 113 |
Ch. 10 | Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity | 123 |
Ch. 11 | Automatic Karyotype Analysis | 141 |
Ch. 12 | Bivariate Chromosome Analysis Using a Commercial Flow Cytometer | 187 |
Ch. 13 | Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping | 205 |
Ch. 14 | Electrophoretic Karyotype Analysis: Pulsed Field Gel Electrophoresis | 221 |
Ch. 15 | Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations | 253 |
Ch. 16 | Immunocytochemical Techniques Applied to Meiotic Chromosomes | 287 |
Ch. 17 | Immortalized Cell Lines: Their Creation and Use in Gene Mapping | 303 |
Ch. 18 | Oligonucleotide Primed in Situ DNA Synthesis (PRINS): An Alternative to in Situ Hybridization for Gene Mapping and the Inversion of Genome Organization | 323 |
Ch. 19 | Electron Microscopic Localization of in Situ Hybrids | 335 |
Ch. 20 | Protocols for Chromosome-Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping | 353 |
Ch. 21 | Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines | 379 |
Ch. 22 | Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints | 403 |
Ch. 23 | Chromosome Dissection and Cloning | 425 |
Ch. 24 | Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization | 437 |
Ch. 25 | Molecular Analysis of Chromosome Aberrations: In Situ Hybridization | 449 |
Ch. 26 | Alu- and L1-Primed PCR-Generated Probes for Nonisotopic in Situ Hybridization | 479 |
Ch. 27 | Chromosome Substructure Investigation: Telomeres | 493 |
Index | 505 |
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Add Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols to the inventory that you are selling on WonderClubX
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Add Chromosome Analysis Protocols, Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to othe, Chromosome Analysis Protocols to your collection on WonderClub |