Metal Ions in Biological Systems: Applications of Magnetic Resonance to Paramagnetic Species, Vol. 21 Book

	Preface to the Series	iii
	Preface to Volume 21	v
	Contributors	xi
	Contents of Other Volumes	xiii
Chapter 1	Nuclear Relaxation Times as a Source of Structural Information	1
1.	Theoretical Considerations	2
2.	Applications	19
	Abbreviations	41
	References	41
Chapter 2	Nuclear Relaxation in NMR of Paramagnetic Systems	47
1.	Nuclear Relaxation and Observability of the NMR Signals	48
2.	Physical Consequences of Unpaired Electron-Nucleus Coupling	51
3.	T[superscript -1 subscript 1M] and Nuclear Magnetic Relaxation Dispersion (NMRD)	60
4.	T[superscript -1 subscript 2M]	67
5.	Electron Relaxation Mechanisms	69
6.	Electron and Nuclear Relaxation in Magnetically Coupled Systems	72
7.	Concluding Remarks	81
	Abbreviations	82
	References	82
Chapter 3	NMR Studies of Magnetically Coupled Metalloproteins	87
1.	Introduction	88
2.	Physical Background	88
3.	Superoxide Dismutase	91
4.	Hemerythrin	97
5.	Ribonucleotide Reductase	107
6.	Uteroferrin	108
7.	Ferredoxins	113
8.	Summary	114
	Abbreviations	115
	References	116
Chapter 4	Proton NMR Studies of Biological Problems Involving Paramagnetic Heme Proteins	121
1.	Heme Proteins	122
2.	The Hyperfine Shift in Paramagnetic Heme Proteins	132
3.	Resonances of the Porphyrin Ring	144
4.	Resonances of Axial Ligands	161
5.	Resonances of the Heme Pocket not Attributable to Heme Ligands	168
6.	Proton NMR Studies of Protein-Protein Complexes	171
7.	Conclusion	177
	Abbreviations	178
	References	179
Chapter 5	Metal-Porphyrin Induced NMR Dipolar Shifts and Their Use in Conformational Analysis	187
1.	Introduction	188
2.	Theory	188
3.	Structural and Magnetic Properties of Metalloporphyrins	194
4.	Conformational Analysis	200
5.	Conclusions and Outlook	221
	Abbreviations	223
	References	223
Chapter 6	Relaxometry of Paramagnetic Ions in Tissue	229
1.	General Background	230
2.	Relaxometry of Fe[superscript 3+]	233
3.	Relaxometry of Mn[superscript 2+]	243
4.	Relaxometry of Gd[superscript 3+]	251
5.	The Present and Future	261
6.	Concluding Remarks	267
	Abbreviations	267
	References	268
	Author Index	271
	Subject Index	285