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Calcium Signalling: A Practical Approach Book

Calcium Signalling: A Practical Approach
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  • Calcium Signalling: A Practical Approach
  • Written by author Alexei Tepikin
  • Published by Oxford University Press, USA, January 2001
  • This new edition focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators
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Authors

Preface
List of protocols
Abbreviations
Pt. 1New probes, new instruments, new methods
1Cameleons as cytosolic and intra-organeller calcium probes3
2Photometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studies17
3Detecting and minimizing errors in calcium-probe measurements arising from transition metals and zinc45
4Targeting of bioluminescent probes and calcium measurements in different subcellular compartments59
Pt. 2Calcium measurement in different organelles
5Monitoring mitochondrial function in single cells79
6Using low-affinity fluorescent calcium indicators and chelators for monitoring and manipulating free [Ca[superscript 2+]] in the endoplasmic reticulum111
7Measuring calcium in the nuclear envelope and nucleoplasm125
Pt. 3Monitoring specific calcium reactions
8Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells139
9Monitoring the generation and propagation of calcium signals in multicellular preparations155
10Pharmacological studies of new calcium release mechanisms177
11Measuring calcium extrusion197
A1Fluorescent calcium indicators and caged calcium probes discussed in this volume215
A2: List of suppliers219
Index225


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