Bacterial Pathogenesis(Selected Methods in Enzymology Series), Ba

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List of Contributors
Preface
Contents of Methods in Enzymology Volumes 235 and 236
1 Recommendations for Working with Pathogenic Bacteria 3
2 Determination of Median Lethal and Infectious Doses in Animal Model Systems 29
3 Serogroup and Serotype Classification of Bacterial Pathogens 43
4 Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing 59
5 Determinations of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes 69
6 DNA Fingerprinting of Myobacterium tuberculosis 83
7 Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences 93
8 Isolation of Outer Membranes 113
9 Isolation and Characterization of Lipopolysaccharides, Lipooligosaccharides, and Lipid A 123
10 Isolation of Peptidoglycan and Soluble Peptidoglycan Fragments 135
11 Purification of Streptococcal M Protein 169
12 Purification of Escherichia coli K Antigens 179
13 Purification of Somatic Pili 187
14 Detection, Isolation, and Characterization of Siderophores 201
15 Identification of Receptor-Mediated Transferrin-Iron Uptake Mechanism in Neisseria gonorrhoeae 217
16 Analysis and Construction of Stable Phenotypes in Gram-Negative Bacteria with Tn5- and Tn10-Derived Minitransposons 227
17 Use of Transposons to Dissect Pathogenic Strategies of Gram-Positive Bacteria 247
18 Identification of Bacterial Cell-Surface Virulence Determinants with TnphoA 269
19 Temperature-Sensitive Mutants of Bacterial Pathogens: Isolation and Use to Determine Host Clearance and in Vivo Replication Rates 291
20 Use of Conditionally Counterselectable Suicide Vectors for Allelic Exchange 301
21 In Vivo Expression Technology for Selection of Bacterial Genes Specifically Induced in Host Tissues 309
22 Zymographic Techniques for Detection and Characterization of Microbial Proteases 323
23 ADP-Ribosylating Toxins 355
24 Assays of Hemolytic Toxins 371
25 Assay of Cytopathogenic Toxins in Cultured Cells 383
26 Use of Lipid Bilayer Membranes to Detect Pore Formation by Toxins 395
27 Uptake and Processing of Toxins by Mammalian Cells 411
28 Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products 425
29 Effect of Bacterial Products on Colony-Stimulating Factor Production 433
30 Effect of Bacterial Products of Tumor Necrosis Factor Production: Quantitation in Biological Fluids or Tissues 447
31 Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin 457
32 Effect of Bacterial Products on Interferon Production 467
33 Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes 475
34 Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils 493
35 Measurement of Human Neutrophil Respiratory Burst Activity during Phagocytosis of Bacteria 505
36 Complement-Meditated Bacterial Killing Assays 523
37 Measurement of Phagosome-Lysosome Fusion and Phagosomal pH 535
38 Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing 549
39 Bacterial Lectinlike Adhesins: Determination and Specificity 565
40 Possible Interaction between Animal Lectins and Bacterial Carbohydrates 587
41 Isolation and Identification of Eukaryotic Receptors Promoting Bacterial Internalization 611
42 Interactions of Bacteria with Leukocyte Integrins 623
43 Solid-Phase Binding of Microorganisms to Glycolipids and Phospholipids 635
44 Characterization of Microbial Host Receptors 643
45 Measurement of Invasion by Gentamicin Resistance 667
46 Measurements of Invasion by Antibody Labeling and Electron Microscopy 683
47 Polarized Epithelial Monolayers: Model Systems to Study Bacterial Interactions with Host Epithelial Cells 701
48 Interjunctional Invasion of Endothelial Cell Monolayers 711
49 Intracellular Growth of Bacteria 729
50 Inhibitors of Cytoskeletal Function and Signal Transduction to Study Bacterial Invasion 733
51 Methods to Visualize Actin Polymerization Associated with Bacterial Invasion 743
52 Measurement of Free Intracellular Calcium Levels in Epithelial Cells as Consequence of Bacterial Invasion 749
53 Shigella flexneri: Isolation of Noninvasive Mutants of Gram-Negative Pathogens 761
54 Isolation of Salmonella Mutants Defective for Intracellular Survival 779
55 Small Plaque Mutants 797
Index 803

Title: Bacterial Pathogenesis(Selected Methods in Enzymology Series)

Manufacturer:

Elsevier

Item Number: 9780121754655

Publication Date: September 1997

Number: 1

Product Description: Bacterial Pathogenesis(Selected Methods in Enzymology Series)

Universal Product Code (UPC): 9780121754655

WonderClub Stock Keeping Unit (WSKU): 9780121754655

Rating: 3/5 based on 2 Reviews

Image Location: https://wonderclub.com/images/covers/46/55/9780121754655.jpg

Category: Media >> Books

Weight: 0.200 kg (0.44 lbs)

Width: 7.340 cm (2.89 inches)

Heigh : 9.460 cm (3.72 inches)

Depth: 1.520 cm (0.60 inches)

Date Added: August 25, 2020, Added By: Ross

Date Last Edited: August 25, 2020, Edited By: Ross

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Bacterial Pathogenesis(Selected Methods in Enzymology Series)

\\ddc\user files\carol\books\reviews\calvin, william h - a brain for all seasons.doc
William H. Calvin
June 29th 2003
A Brain for all Seasons
A challenging attempt at an overview of how the human brain and climate change interacted to make us what we are. This book has any fascinating facts and interesting interpretations. Sometimes one wishes the book were longer and in more detail, but there is a bibliography which I will work through.
Many of the theories are contentious, and Calvin generally makes sure the reader is aware of the difference between fact and fiction, unlike many of the TV shows one sees nowadays.
The earlier parts of the book, covering prehistory, are fascinating. The last couple of chapters covering the present are scary, probably very appropriate, and incredibly interventionist in attitude to climate change.
Calvin basically starts from the premise that we have irreversible changed the climate and environment. He agrees that we should try to limit the damage, but argues that there is never was a stable climate or environment in the past, and that there never will be one on the future.
His proposals for intervention (dams in the arctic, blowing up ice barriers and so on) may sound extreme, but if his is right and humankind has developed in one of the most stable periods of the Earth's climatic history, violent climate change is inevitable, whether provoked by human intervention, or caused by the same forces what caused climate change on a vast scale before man was around.
And violent climate change is something we should be modest about, realise we will have very little control over it, and do the best to survive. The scary bit is how ugly survival will be. The "War for Oil" theorists would say we have an example of it already. I don't have the information to judge, but certainly "might is right" has not vanished as a ruling principle.
A Brain for all Seasons
October 2004
This book, which I probably read last year (the beginning of this year was consumed by the Reformation), has me making notes all over the place.
Calvin's thesis is that climate change, both local and global is a trigger for evolution is fascinating and credible. His writing style is perhaps not the greatest, and the travelogue is not my favourite vehicle for presenting scientific theory, but for all that I enjoyed the book greatly.
\\ddc\user files\carol\books\reviews\calvin, william h - a brain for all seasons.doc
I still want to do a project with a visual map of the world evolving over time, with continents, climate information and micro events that are documented all available at the click of a mouse.
I did even go as far as to start collecting data. If time permits I will do more.
M. Calvin earns his living as an academic, and presents a strong case for his theories, some of which are very perceptive and simple. In the end however I think he is somewhat arrogant, and possibly dangerous with his ideas for reducing the damage caused by global warming. His simplistic interventionist solutions could only to well appeal to someone like George W. Bush and lead to an ecological disaster as a half thought through version is poorly implemented and executed.
I won't spoil the fun by telling you what they are, read the book, learn on the way, and to retain your sense of modesty, go and visit a cave with old stalactites with a geologist, and have them run through the history of the world and climate as told by the minerals deposited by the drops of water.

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	List of Contributors
	Preface
	Contents of Methods in Enzymology Volumes 235 and 236
1	Recommendations for Working with Pathogenic Bacteria	3
2	Determination of Median Lethal and Infectious Doses in Animal Model Systems	29
3	Serogroup and Serotype Classification of Bacterial Pathogens	43
4	Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing	59
5	Determinations of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes	69
6	DNA Fingerprinting of Myobacterium tuberculosis	83
7	Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences	93
8	Isolation of Outer Membranes	113
9	Isolation and Characterization of Lipopolysaccharides, Lipooligosaccharides, and Lipid A	123
10	Isolation of Peptidoglycan and Soluble Peptidoglycan Fragments	135
11	Purification of Streptococcal M Protein	169
12	Purification of Escherichia coli K Antigens	179
13	Purification of Somatic Pili	187
14	Detection, Isolation, and Characterization of Siderophores	201
15	Identification of Receptor-Mediated Transferrin-Iron Uptake Mechanism in Neisseria gonorrhoeae	217
16	Analysis and Construction of Stable Phenotypes in Gram-Negative Bacteria with Tn5- and Tn10-Derived Minitransposons	227
17	Use of Transposons to Dissect Pathogenic Strategies of Gram-Positive Bacteria	247
18	Identification of Bacterial Cell-Surface Virulence Determinants with TnphoA	269
19	Temperature-Sensitive Mutants of Bacterial Pathogens: Isolation and Use to Determine Host Clearance and in Vivo Replication Rates	291
20	Use of Conditionally Counterselectable Suicide Vectors for Allelic Exchange	301
21	In Vivo Expression Technology for Selection of Bacterial Genes Specifically Induced in Host Tissues	309
22	Zymographic Techniques for Detection and Characterization of Microbial Proteases	323
23	ADP-Ribosylating Toxins	355
24	Assays of Hemolytic Toxins	371
25	Assay of Cytopathogenic Toxins in Cultured Cells	383
26	Use of Lipid Bilayer Membranes to Detect Pore Formation by Toxins	395
27	Uptake and Processing of Toxins by Mammalian Cells	411
28	Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products	425
29	Effect of Bacterial Products on Colony-Stimulating Factor Production	433
30	Effect of Bacterial Products of Tumor Necrosis Factor Production: Quantitation in Biological Fluids or Tissues	447
31	Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin	457
32	Effect of Bacterial Products on Interferon Production	467
33	Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes	475
34	Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils	493
35	Measurement of Human Neutrophil Respiratory Burst Activity during Phagocytosis of Bacteria	505
36	Complement-Meditated Bacterial Killing Assays	523
37	Measurement of Phagosome-Lysosome Fusion and Phagosomal pH	535
38	Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing	549
39	Bacterial Lectinlike Adhesins: Determination and Specificity	565
40	Possible Interaction between Animal Lectins and Bacterial Carbohydrates	587
41	Isolation and Identification of Eukaryotic Receptors Promoting Bacterial Internalization	611
42	Interactions of Bacteria with Leukocyte Integrins	623
43	Solid-Phase Binding of Microorganisms to Glycolipids and Phospholipids	635
44	Characterization of Microbial Host Receptors	643
45	Measurement of Invasion by Gentamicin Resistance	667
46	Measurements of Invasion by Antibody Labeling and Electron Microscopy	683
47	Polarized Epithelial Monolayers: Model Systems to Study Bacterial Interactions with Host Epithelial Cells	701
48	Interjunctional Invasion of Endothelial Cell Monolayers	711
49	Intracellular Growth of Bacteria	729
50	Inhibitors of Cytoskeletal Function and Signal Transduction to Study Bacterial Invasion	733
51	Methods to Visualize Actin Polymerization Associated with Bacterial Invasion	743
52	Measurement of Free Intracellular Calcium Levels in Epithelial Cells as Consequence of Bacterial Invasion	749
53	Shigella flexneri: Isolation of Noninvasive Mutants of Gram-Negative Pathogens	761
54	Isolation of Salmonella Mutants Defective for Intracellular Survival	779
55	Small Plaque Mutants	797
	Index	803